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Induction of Myocardial Infarction in Adult Zebrafish Using Cryoinjury
Journal JoVE
Médecine
This content is Free Access.
Journal JoVE Médecine
Induction of Myocardial Infarction in Adult Zebrafish Using Cryoinjury
DOI:

11:12 min

April 18, 2012

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Chapitres

  • 00:05Titre
  • 02:30Equipment Set-up
  • 03:18The Cryoinjury Procedure
  • 05:37Heart Collection and Fixation
  • 06:33Heart Mounting
  • 07:29Heart Sectioning
  • 08:41Representative Cryoinjury Results
  • 10:22Conclusion

Summary

Traduction automatique

Zebrafish represents a valuable model to study the mechanisms of heart regeneration in vertebrates. Here, we present a protocol for induction of a heart infarct in adult zebrafish using cryoinjury. This method results in massive cell death within 20% of the ventricular wall, similar to that observed in mammalian infarcts.

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