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Chapters
- 00:05Title
- 02:30Equipment Set-up
- 03:18The Cryoinjury Procedure
- 05:37Heart Collection and Fixation
- 06:33Heart Mounting
- 07:29Heart Sectioning
- 08:41Representative Cryoinjury Results
- 10:22Conclusion
Zebrafish represents a valuable model to study the mechanisms of heart regeneration in vertebrates. Here, we present a protocol for induction of a heart infarct in adult zebrafish using cryoinjury. This method results in massive cell death within 20% of the ventricular wall, similar to that observed in mammalian infarcts.
