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Bioengineering

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JoVE Journal Bioengineering
Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery
 

Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery

Article DOI: 10.3791/3876-v • 13:00 min • April 17th, 2012
April 17th, 2012 •
1,2, 1, 1, 1, 1, 1, 1
1Department of Nanomedicine, The Methodist Hospital Research Institute, 2CAS Key Laboratory for Biological Effects of Nanomaterials & Nanosafety, National Center for Nanoscience and Technology

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résumé
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We developed a technology based on mesoporous silica thin film for the selective recovery of low molecular weight proteins and peptides from human serum. The physico-chemical properties of our mesoporous chips were finely tuned to provide substantial control in peptide enrichment and consequently profile the serum proteome for diagnostic purposes.

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Low Molecular Weight Protein Enrichment Mesoporous Silica Thin Films Biomarker Discovery Circulating Biomarkers Early Diagnosis Prognosis Therapeutic Efficiency Low Molecular Weight Proteome (LMWP) Mass Spectrometry (MS) Dynamic Range Protein And Peptide Concentrations Sample Pre-treatment Highly Abundant Proteins Low-abundance Species Proteomic-based Approaches Two-dimensional Polyacrylamide Gel-electrophoresis (2D-PAGE) Shotgun Proteomics Methods Clinical Applications Isolation Of LMWP From Blood High Throughput Screening Multi-fractionation System Mesoporous Silica Chips
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