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JoVE Journal Bioengineering
Design and Construction of Artificial Extracellular Matrix (aECM) Proteins from Escherichia coli for Skin Tissue Engineering
 

Design and Construction of Artificial Extracellular Matrix (aECM) Proteins from Escherichia coli for Skin Tissue Engineering

Article DOI: 10.3791/52845-v • 10:30 min • June 11th, 2015
June 11th, 2015 •
1, 1, 1
1School of Materials Science and Engineering, Nanyang Technological University

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Recombinant technologies have enabled material designers to create novel artificial proteins with customized functionalities for tissue engineering applications. For example, artificial extracellular matrix proteins can be designed to incorporate structural and biological domains derived from native ECMs. Here, we describe the construction and purification of aECM proteins containing elastin-like repeats.

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Artificial Extracellular Matrix (aECM) Proteins Escherichia Coli Skin Tissue Engineering Recombinant Technology Functional Domains Fibronectin Laminins Collagens Elastin-like Repeats Silk Collagen Repeats Tissue Engineering Wound Repair Integrin-specific AECM Proteins Human Skin Keratinocyte Attachment And Propagation Cell Binding Domains Elastin-like Repeats Substrates In Artificial Skin Design And Construction Of AECM Proteins Purification Process Thermo-responsive Characteristics Of Elastin
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