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Bioengineering

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JoVE Journal Bioengineering
Harvesting Murine Alveolar Macrophages and Evaluating Cellular Activation Induced by Polyanhydride Nanoparticles
 

Harvesting Murine Alveolar Macrophages and Evaluating Cellular Activation Induced by Polyanhydride Nanoparticles

Article DOI: 10.3791/3883-v • 11:26 min • June 8th, 2012
June 8th, 2012 •
1, 2, 2, 2, 1
1Department of Chemical and Biological Engineering, Iowa State University, 2Department of Veterinary Microbiology and Preventive Medicine, Iowa State University

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Herein, we describe protocols for harvesting murine alveolar macrophages, which are resident innate immune cells in the lung, and examining their activation in response to co-culture with polyanhydride nanoparticles.

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Murine Alveolar Macrophages Cellular Activation Polyanhydride Nanoparticles Intranasal Vaccines Respiratory Infectious Diseases Sebacic Acid 1,6-bis(p-carboxyphenoxy)hexane 1,8-bis(p-carboxyphenoxy)-3,6-dioxaoctane Bulk Erosion Kinetics Surface Erosion Kinetics Biomolecules Release Adjuvant Activity Vaccine Delivery Platform Innate Immunity Molecular Responses Cellular Responses Antigen Presenting Cells (APCs) Dendritic Cells Alveolar Macrophages (AM) Lung Parenchyma Microbial Pathogens Cell Debris
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