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Bioengineering

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JoVE Journal Bioengineering
Analyzing Cellular Internalization of Nanoparticles and Bacteria by Multi-spectral Imaging Flow Cytometry
 
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Analyzing Cellular Internalization of Nanoparticles and Bacteria by Multi-spectral Imaging Flow Cytometry

Article DOI: 10.3791/3884-v • 18:07 min • June 8th, 2012
June 8th, 2012 •
1, 1, 2, 3, 1, 1, 1, 3, 1, 1
1Department of Veterinary Microbiology and Preventive Medicine, Iowa State University, 2Amnis Corporation, 3Department of Chemical and Biological Engineering, Iowa State University

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In this article, we describe a method utilizing multi-spectral imaging flow cytometry to quantify the internalization of polyanhydride nanoparticles or bacteria by RAW 264.7 cells.

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Cellular Internalization Nanoparticles Bacteria Multi-spectral Imaging Flow Cytometry Vaccine Delivery Antigen Presenting Cells Nanoparticle Formulation High Throughput Quantitative Experimental Protocol Microscopy Flow Cytometry Phagocytosis Statistical Data Spatial Resolution Multi-color Spectral Fluorescence Bright Field Imaging
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