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JoVE Journal Medicine
Collection Protocol for Human Pancreas
 

Collection Protocol for Human Pancreas

Article DOI: 10.3791/4039-v • 07:49 min • May 23rd, 2012
May 23rd, 2012 •
1, 1, 1, 1, 1, 1, 1
1Department of Pathology, Immunology, and Laboratory Medicine, University of Florida

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résumé
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This video demonstrates a dissection procedure for processing human pancreas into multiple storage formats. Anatomical orientation is maintained throughout the pancreatic regions to allow definition of regional islet composition and density.

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Collection Protocol Human Pancreas Dissection Sampling Procedure Network For Pancreatic Organ Donors With Diabetes (nPOD) Standardize Cadaveric Organ Donors Pancreas Regions Head Body Tail Serial Transverse Sections Fixed Paraffin Fresh Frozen Blocks Snap Frozen Sample Preparations RNAse Inhibitors DNA RNA Protein Isolation Pancreas Dissection Procedure Anatomical Orientation Endocrine Cell Heterogeneity Islet Composition Size Numbers Insulin-containing β-cells Glucagon-containing α-cells Somatostatin-containing δ-cells Pancreatic Polypeptide-containing PP Cells Ghrelin-containing Epsilon Cells Uncinate Region Developmental Differences
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