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Neo-Islet Formation in Liver of Diabetic Mice by Helper-dependent Adenoviral Vector-Mediated Gene Transfer
 

Neo-Islet Formation in Liver of Diabetic Mice by Helper-dependent Adenoviral Vector-Mediated Gene Transfer

Article DOI: 10.3791/4321-v • 16:59 min • October 10th, 2012
October 10th, 2012 •
1,2, 1,2,3, 1,2,3
1Department of Medicine, Baylor College of Medicine, 2Division of Diabetes, Endocrinology & Metabolism, Diabetes & Endocrinology Research Center, Baylor College of Medicine, 3Department of Molecular & Cellular Biology, Baylor College of Medicine

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résumé
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We describe hepatic neo-islet formation in STZ (streptozotocin)-induced diabetic mice by gene transfer of Neurogenin3 (Ngn3) and Betacellulin (Btc) using helper-dependent adenoviral vector (HDAd) and the reversal of hyperglycemia. Our method takes advantages of helper-dependent adenoviral vectors with their highly efficient in vivo transduction and the long lasting gene expression.

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Neo-Islet Formation Liver Diabetic Mice Helper-dependent Adenoviral Vector-Mediated Gene Transfer Type 1 Diabetes T Cell-mediated Autoimmune Destruction Insulin-producing Cells Pancreas Insulin Replacement Islet Transplantation Donor Availability Long-term Immunosuppression Induced Islet Neogenesis Gene Transfer Neuogenin3 (Ngn3) Islet Lineage-defining Specific Transcription Factor Betacellulin (Btc) Islet Growth Factor Adenoviral Vectors (Ads) Gene Transfer Vector Early Generation Ads Safety Profile Transgene Expression Chronic Toxicity Viral Coding Sequences Ad Cis Elements Vector Replication And Packaging Protocol HDAd-Ngn3 HDAd-Btc STZ-induced Diabetic Mice 'neo Islets' Liver Hyperglycemia
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