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Neuroscience

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JoVE Journal Neuroscience
Simultaneous Whole-cell Recordings from Photoreceptors and Second-order Neurons in an Amphibian Retinal Slice Preparation
 

Simultaneous Whole-cell Recordings from Photoreceptors and Second-order Neurons in an Amphibian Retinal Slice Preparation

Article DOI: 10.3791/50007-v • 11:39 min • June 1st, 2013
June 1st, 2013 •
1, 1,2
1Department of Ophthalmology and Visual Sciences, University of Nebraska Medical Center, 2Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center

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We describe the preparation of thin retinal slices from aquatic tiger salamanders (Ambystoma tigrinum) and explain how we use these slices to study synaptic processing in the retina by obtaining dual whole-cell voltage clamp recordings from photoreceptors and second-order horizontal and bipolar cells.

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Keywords: Retinal Neuroscience Retinal Circuitry Photoreceptors Retinal Neurons Electrophysiological Recording Vertical Retinal Slice Dual Whole-cell Voltage Clamp Photoreceptor Membrane Potential Synaptic Ribbon Neurotransmitter Release
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