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Immunology and Infection

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JoVE Journal Immunology and Infection
Clinical Application of Sleeping Beauty and Artificial Antigen Presenting Cells to Genetically Modify T Cells from Peripheral and Umbilical Cord Blood
 

Clinical Application of Sleeping Beauty and Artificial Antigen Presenting Cells to Genetically Modify T Cells from Peripheral and Umbilical Cord Blood

Article DOI: 10.3791/50070-v • 09:29 min • February 1st, 2013
February 1st, 2013 •
1, 1, 1, 1, 2, 2, 2, 1, 1
1Division of Pediatrics, U.T. MD Anderson Cancer Center, 2Department of Stem Cell Transplantation and Cellular Therapy, U.T. MD Anderson Cancer Center

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résumé
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T cells expressing a CD19-specific chimeric antigen receptor (CAR) are infused as investigational treatment of B-cell malignancies in our first-in-human gene therapy trials. We describe genetic modification of T cells using the Sleeping Beauty (SB) system to introduce CD19-specific CAR and selective propagation on designer CD19+ artificial antigen presenting cells.

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Sleeping Beauty Artificial Antigen Presenting Cells T Cells Gene Therapy Immunotherapy Tumor-associated Antigens CAR (Chimeric Antigen Receptor) Retrovirus Lentivirus Transduction Nonviral Plasmids Clinical Grade Recombinant Virus Transposon Transposase CD19-specific CAR Transgene
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