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Immunology and Infection

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JoVE Journal Immunology and Infection
Isolation and Adoptive Transfer of High Salt Treated Antigen-presenting Dendritic Cells
 

Isolation and Adoptive Transfer of High Salt Treated Antigen-presenting Dendritic Cells

Article DOI: 10.3791/59124-v • 09:29 min • March 5th, 2019
March 5th, 2019 •
1, 1, 1, 1, 1
1Division of Clinical Pharmacology, Department of Medicine, Vanderbilt University Medical Center

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Automatic Translation

Here, we present a protocol to isolate dendritic cells from murine spleens by magnetic cell sorting and subsequent adoptive transfer into naïve mice. The model of high-salt activated dendritic cells was chosen to explain the step-by-step procedures of adoptive transfer and flow cytometry.

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Keywords: Dendritic Cells CD11c Hypertension Cardiovascular Disease Adoptive Transfer Spleen Isolation Collagenase D DNase MACS Flow Cytometry
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