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Immunology and Infection

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JoVE Journal Immunology and Infection
Analysis of the Solvent Accessibility of Cysteine Residues on Maize rayado fino virus Virus-like Particles Produced in Nicotiana benthamiana Plants and Cross-linking of Peptides to VLPs
 

Analysis of the Solvent Accessibility of Cysteine Residues on Maize rayado fino virus Virus-like Particles Produced in Nicotiana benthamiana Plants and Cross-linking of Peptides to VLPs

Article DOI: 10.3791/50084-v • 08:27 min • February 14th, 2013
February 14th, 2013 •
1,2, 1,2
1Plant Sciences Institute, Agricultural Research Service, United States Department of Agriculture, 2Molecular Plant Pathology Laboratory, Agricultural Research Service, United States Department of Agriculture

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A method to analyze the solvent accessibility of the thiol group of cysteine residues of Maize rayado fino virus (MRFV)-virus-like particles (VLPs) followed by a peptide cross-linking reaction is described. The method takes advantage of the availability of several chemical groups on the surface of the VLPs that can be targets for specific reactions.

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Keywords: Virus-like Particles Maize Rayado Fino Virus Solvent Accessibility Cysteine Residues Peptide Bioconjugation Virus Modification Virus-based Technologies Plant-produced VLPs Virus Structure And Properties
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