Journal
/
Neurosciences
/
A Method for High Fidelity Optogenetic Control of Individual Pyramidal Neurons In vivo
Journal JoVE
Neurosciences
This content is Free Access.
Journal JoVE Neurosciences
A Method for High Fidelity Optogenetic Control of Individual Pyramidal Neurons In vivo

A Method for High Fidelity Optogenetic Control of Individual Pyramidal Neurons In vivo

DOI:
10.3791/50291-v

•

13:44 min

•

September 02, 2013

•

, ,
1Institute for Behavioral Genetics,University of Colorado Boulder, 2Department of Psychology and Neuroscience,University of Colorado Boulder

Chapitres

  • 00:05Titre
  • 01:50Virus Storage and Preparation
  • 02:57Surgery and Virus Injection
  • 04:59Light Delivery and In vivo Recording of CHR2 or NpHR-Expressing Neurons
  • 08:38Results: High Fidelity Optogenetic Control of Individual Pyramidal Neurons In vivo
  • 13:06Conclusion

Summary

Traduction automatique

Traduction automatique

The recent development of neuroscience tools that combine genetics and optics, termed "optogenetics", enables control over neural circuit activity with an unprecedented level of spatial and temporal resolution. Here we provide a protocol for integrating in vivo recording with optogenetic manipulation of genetically-defined subsets of prefrontal cortical and subicular pyramidal neurons.

Tags

OptogeneticsNeural CircuitChannelrhodopsin-2HalorhodopsinAdeno-associated VirusDorsal SubiculumPrelimbic RegionPrefrontal CortexPyramidal NeuronIn VivoElectrical ActivityLight DeliveryElectrical Recording

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/50291?article_type=v

Mechanical Manipulation of Neurons to Control Axonal Development
check_url/fr/50291?article_type=v

In vivo Electroporation of Developing Mouse Retina
check_url/fr/50291?article_type=v

Selective Viral Transduction of Adult-born Olfactory Neurons for Chronic in vivo Optogenetic Stimulation
check_url/fr/50291?article_type=v

Retrograde Fluorescent Labeling Allows for Targeted Extracellular Single-unit Recording from Identified Neurons In vivo
check_url/fr/50291?article_type=v

In vivo Optogenetic Stimulation of the Rodent Central Nervous System
check_url/fr/50291?article_type=v

Ex Vivo Optogenetic Dissection of Fear Circuits in Brain Slices
check_url/fr/50291?article_type=v

A Simplified Method for Ultra-Low Density, Long-Term Primary Hippocampal Neuron Culture
check_url/fr/50291?article_type=v

Optical Control of a Neuronal Protein Using a Genetically Encoded Unnatural Amino Acid in Neurons
check_url/fr/50291?article_type=v

Ratiometric Calcium Imaging of Individual Neurons in Behaving Caenorhabditis Elegans
check_url/fr/50291?article_type=v

Method for High Speed Stretch Injury of Human Induced Pluripotent Stem Cell-derived Neurons in a 96-well Format

Read Article