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Bioengineering

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JoVE Journal Bioengineering
Creating Adhesive and Soluble Gradients for Imaging Cell Migration with Fluorescence Microscopy
 

Creating Adhesive and Soluble Gradients for Imaging Cell Migration with Fluorescence Microscopy

Article DOI: 10.3791/50310-v • 13:10 min • April 4th, 2013
April 4th, 2013 •
1, 1, 2, 1, 1, 2, 1, 1
1Centre for Vascular Research and Australian Centre for Nanomedicine, The University of New South Wales, 2School of Chemistry and Australian Centre for Nanomedicine, The University of New South Wales

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résumé
Automatic Translation

A method for the assembly of adhesive and soluble gradients in a microscopy chamber for live cell migration studies is described. The engineered environment combines antifouling surfaces and adhesive tracks with solution gradients and therefore allows one to determine the relative importance of guidance cues.

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Adhesive Gradients Soluble Gradients Cell Migration Fluorescence Microscopy Microfluidic Chamber Live Cell Imaging Copolymer Poly-L-lysine Polyethylene Glycol Passivation Non-specific Adsorption Biomolecules Cells Microcontact Printing Dip Pen Lithography Streptavidin Biotinylated Peptide Arginine-glycine-aspartic Acid (RGD) Microfluidic Device Chemoattractant Fetal Bovine Serum (FBS)
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