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JoVE Journal Neuroscience
Genetic Manipulation of the Mouse Developing Hypothalamus through In utero Electroporation
 

Genetic Manipulation of the Mouse Developing Hypothalamus through In utero Electroporation

Article DOI: 10.3791/50412-v • 11:48 min • July 24th, 2013
July 24th, 2013 •
*1, *2, 1, 1
1Institute of Anatomy and Cell Biology, University of Heidelberg, 2Institut de recherches cliniques de Montreal
* These authors contributed equally

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résumé
Automatic Translation

Despite the functional and medical importance of the hypothalamus, in utero genetic manipulation of its development has rarely been attempted. We show a detailed procedure for in utero electroporation into the mouse hypothalamus and show representative results of total and partial (regional) hypothalamic transfection.

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Keywords: Genetic Manipulation Mouse Developing Hypothalamus In Utero Electroporation Genetic Modification Mammalian Brain Mouse Mutants Developing Brain Cortex Thalamus Hypothalamus Hypothalamic Neuroepithelium Mammillary Body Electroporation
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