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Neuroscience

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JoVE Journal Neuroscience
Micromanipulation of Gene Expression in the Adult Zebrafish Brain Using Cerebroventricular Microinjection of Morpholino Oligonucleotides
 

Micromanipulation of Gene Expression in the Adult Zebrafish Brain Using Cerebroventricular Microinjection of Morpholino Oligonucleotides

Article DOI: 10.3791/50415-v • 05:48 min • May 23rd, 2013
May 23rd, 2013 •
1, 1, 1, 1
1DFG-Center for Regenerative Therapies Dresden, Cluster of Excellence (CRTD) and Biotechnology Center (BIOTEC) of the Technische Universität Dresden

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In this article, we demonstrate a method for manipulation of gene expression in the ventricular cells of the adult zebrafish telencephalon using antisense morpholino oligonucleotides. We present this method as an efficient and quick protocol that can be used for functional studies in the adult vertebrate brain.

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Keywords: Gene Expression Adult Zebrafish Brain Cerebroventricular Microinjection Morpholino Oligonucleotides Radial Glial Cells Neurogenesis Neurodegeneration Central Nervous System Regeneration
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