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Journal / Bioengineering / CometChip: A High-throughput 96-Well Platform for …
JoVE Journal
Bioengineering

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CometChip: A High-throughput 96-Well Platform for Measuring DNA Damage in Microarrayed Human Cells

Article DOI: 10.3791/50607-v • 10:59 min • October 18th, 2014
October 18th, 2014 •
*1, *2, 3, 1, 1, 2, 2, 1
1Department of Biological Engineering, Massachusetts Institute of Technology, 2Environmental Toxicology, Chulabhorn Graduate Institute, 3Department of Biomedical Engineering, University of Minnesota
* These authors contributed equally

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résumé
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We describe here a platform that allows comet assay detection of DNA damage with unprecedented throughput. The device patterns mammalian cells into a microarray and enables parallel processing of 96 samples. The approach facilitates analysis of base level DNA damage, exposure-induced DNA damage and DNA repair kinetics.

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CometChip High-throughput Platform 96-well Format DNA Damage Microarrayed Human Cells DNA Damaging Agents Aging Disease Cancer Environmental Agents Cellular Metabolites Cancer Treatment Quantify DNA Damage Responses Public Health Pharmaceutical Domain Clinical Domain Microfabrication Techniques Fixed Microarray Single Cell Gel Electrophoresis Assay Comet Assay DNA Migration Matrix Electrical Field Abasic Sites Crosslinks Strand Breaks Agarose Microwell Array Parallel Processing Genotoxic Agents Cellular Repair Response
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