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Bioengineering

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JoVE Journal Bioengineering
Production of Large Numbers of Size-controlled Tumor Spheroids Using Microwell Plates
 

Production of Large Numbers of Size-controlled Tumor Spheroids Using Microwell Plates

Article DOI: 10.3791/50665-v • 10:44 min • November 18th, 2013
November 18th, 2013 •
1, 1, 1
1Department of Comparative Biology & Experimental Medicine, Faculty of Veterinary Medicine, University of Calgary

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résumé
Automatic Translation

We introduce a protocol for the generation of large numbers (thousands to hundreds of thousands) of uniform size- and composition-controlled tumor spheroids, using commercially available microwell plates.

Tags

Tumor Spheroids In Vitro Model Behavior Of Tumor Cells Physiologically Relevant Adherent-sheet Cultures Recapitulate Complexity Interactions In Tumors Generate Spheroids Reproducibly Controlled Manner Significant Numbers AggreWell System Pyramid-shaped Microwells Single Cells Suspension Clustering Of Cells Arbitrary Size And Composition Platform Technology High Production Levels Nonlabor-intensive Process Tumor Spheroid Field
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