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Immunology and Infection

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JoVE Journal Immunology and Infection
Quantitative In vitro Assay to Measure Neutrophil Adhesion to Activated Primary Human Microvascular Endothelial Cells under Static Conditions
 

Quantitative In vitro Assay to Measure Neutrophil Adhesion to Activated Primary Human Microvascular Endothelial Cells under Static Conditions

Article DOI: 10.3791/50677-v • 11:22 min • August 23rd, 2013
August 23rd, 2013 •
1, 1,2, 1
1Department of Anesthesia and Perioperative Care, University of California, San Francisco, 2Graduate Program in Biomedical Sciences, University of California, San Francisco

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Neutrophil adherence to the activated endothelium at sites of infection is an integral component of the host's inflammatory response. Described in this report is a neutrophil binding assay that allows for the in vitro quantitation of primary human neutrophil binding to endothelial cells activated by inflammatory mediators under static conditions.

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Quantitative In Vitro Assay Neutrophil Adhesion Activated Primary Human Microvascular Endothelial Cells Static Conditions Vascular Endothelium Inflammatory Response Acute Phase Of Inflammation Endothelial Cells Host Mediators Microbial Components Danger Molecules Cytokines Chemokines Adhesion Molecules Leukocytes Site Of Infection Or Injury Neutrophils Adhesion Molecules Eliminate Microbial Threats Recruitment Of Leukocytes Wound Repair Recruitment And Attachment To Endothelium Initiation Of Inflammatory Response In Vitro Neutrophil Adhesion Assay Calcein AM-labeled Primary Human Neutrophils Microvascular Endothelial Cell Activation Fluorescence Spectrophotometry Fluorescence Microscopy
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