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Intraductal Injection for Localized Drug Delivery to the Mouse Mammary Gland
 

Intraductal Injection for Localized Drug Delivery to the Mouse Mammary Gland

Article DOI: 10.3791/50692-v • 06:39 min • October 4th, 2013
October 4th, 2013 •
1, 2, 1,2,3
1Vascular Biology Program, Department of Surgery, Boston Children's Hospital and Harvard Medical School, 2Wyss Institute for Biologically Inspired Engineering, Harvard University, 3Harvard School of Engineering and Applied Sciences

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résumé
Automatic Translation

A protocol for the non-invasive intraductal delivery of aqueous reagents to the mouse mammary gland is described. The method takes advantage of localized injection into the nipples of mammary glands targeting mammary ducts specifically. This technique is adaptable for a variety of compounds including siRNA, chemotherapeutic agents and small molecules.

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Intraductal Injection Localized Drug Delivery Mouse Mammary Gland Reagents Targeting Molecules Knock-down Of Genes Mammary Epithelium Pregnancy And Lactation Technique Adulthood Nipples Of Mice Live Mice Anesthesia Non-invasive Localized Drug Delivery Repeated Injections Evans Blue Dye Ductal Tree Visibility Fluorescently Labeled Reagents Visualization Distribution Within Mammary Gland SiRNA Chemotherapeutic Agents Small Molecules
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