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Immunology and Infection

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JoVE Journal Immunology and Infection
Identifiera DNA Mutationer i renat Hematopoietic Stem / progenitorceller
 
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Identifiera DNA Mutationer i renat Hematopoietic Stem / progenitorceller

Article DOI: 10.3791/50752-v • 11:06 min • February 24th, 2014
February 24th, 2014 •
1, 1,2, 1, 3, 4,5, 2, 2,5, 1,2,5
1Greehey Children's Cancer Research Institute, UT Health Science Center at San Antonio, 2Department of Cellular and Structural Biology, UT Health Science Center at San Antonio, 3Department of Pathology, UT Health Science Center at San Antonio, 4Department of Microbiology, UT Health Science Center at San Antonio, 5Cancer Therapy and Research Center, UT Health Science Center at San Antonio

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Här beskriver vi en in vivo-mutagenes-analys för mindre antal renade hematopoetiska celler med Lacl transgen musmodell. Lacl-genen kan isoleras för att bestämma frekvens, plats och typ av DNA-mutanter som spontant uppstått eller efter exponering för genotoxins.

Tags

Infektion , hematopoetiska stamceller / progenitorceller, DNA-mutationer,
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