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Enrichment and Purging of Human Embryonic Stem Cells by Detection of Cell Surface Antigens Using the Monoclonal Antibodies TG30 and GCTM-2
 

Enrichment and Purging of Human Embryonic Stem Cells by Detection of Cell Surface Antigens Using the Monoclonal Antibodies TG30 and GCTM-2

Article DOI: 10.3791/50856-v • 12:43 min • December 6th, 2013
December 6th, 2013 •
1, 1, 1, 1, 1, 1
1Materials Science and Engineering, CSIRO

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We describe the use of the monoclonal antibodies TG30 (CD9) and GCTM-2 for the combined detection of cell surface antigens via fluorescence activated cell sorting (FACS) for the identification and enrichment of live human embryonic stem cells (hESC) using positive selection and also the use of negative selection to purge hESCs from a mixed cell population.

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Enrichment Purging Human Embryonic Stem Cells Cell Surface Antigens Monoclonal Antibodies TG30 GCTM-2 Self-renewal Differentiation Somatic Cell Lineages Transplantation Therapies Cell-degenerative Diseases Pluripotent Differentiated Cells Undifferentiated Cells Stem Cell-free Populations Teratomas Fluorescence Activated Cell Sorting (FACS) Positive Selection Negative Selection
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