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Single-plant, Sterile Microcosms for Nodulation and Growth of the Legume Plant Medicago truncatula with the Rhizobial Symbiont Sinorhizobium meliloti
 

Single-plant, Sterile Microcosms for Nodulation and Growth of the Legume Plant Medicago truncatula with the Rhizobial Symbiont Sinorhizobium meliloti

Article DOI: 10.3791/50916-v • 20:01 min • October 1st, 2013
October 1st, 2013 •
1, *1, *1
1Department of Biological Science, Florida State University
* These authors contributed equally

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Growth of Medicago truncatula plants in symbiosis with the nitrogen-fixing bacteria Sinorhizobium meliloti in individual, sterile microcosms made from standard laboratory plates permits frequent examination of root systems and nodules without compromising sterility. Plants can be maintained in these growth chambers for up to 9 weeks.

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Keywords: Medicago Truncatula Sinorhizobium Meliloti Rhizobial Symbiosis Nitrogen Fixation Nodulation Single-plant Microcosms Non-destructive Imaging Long-term Growth Sterile Conditions Symbiotic Phenotypes
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