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Submillisecond Conformational Changes in Proteins Resolved by Photothermal Beam Deflection
 

Submillisecond Conformational Changes in Proteins Resolved by Photothermal Beam Deflection

Article DOI: 10.3791/50969-v • 10:02 min • February 18th, 2014
February 18th, 2014 •
1, 1
1Department of Chemistry and Biochemistry, Florida International University

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résumé
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Here we report an application of the photothermal beam deflection technique in combination with a caged calcium compound, DM-nitrophen, to monitor microsecond and millisecond dynamics and energetics of structural changes associated with the calcium association to a neuronal calcium sensor, Downstream Regulatory Element Antagonist Modulator.

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Keywords: Photothermal Beam Deflection Photo-acoustic Calorimetry Thermal Grating Conformational Changes Proteins Microsecond To Millisecond Time-scales Volume Changes Enthalpy Changes Calcium Binding Calcium Transducers Neuronal Calcium Sensors DM-nitrophen Photo-triggering Fast Kinetics
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