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A New Approach for the Comparative Analysis of Multiprotein Complexes Based on 15N Metabolic Labeling and Quantitative Mass Spectrometry
 

A New Approach for the Comparative Analysis of Multiprotein Complexes Based on 15N Metabolic Labeling and Quantitative Mass Spectrometry

Article DOI: 10.3791/51103-v • 08:04 min • March 13th, 2014
March 13th, 2014 •
1, 1, 1,2, 1
1Institute of Plant Biology and Biotechnology, University of Münster, 2Department of Plant Biology, Carnegie Institution for Science

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The described comparative, quantitative proteomic approach aims at obtaining insights into the composition of multiprotein complexes under different conditions and is demonstrated by comparing genetically different strains. For quantitative analysis equal volumes of different fractions from a sucrose density gradient are mixed and analyzed by mass spectrometry.

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Keywords: 15N Metabolic Labeling Quantitative Mass Spectrometry Multiprotein Complex Analysis Thylakoid Membrane Cyclic Electron Flow Chlamydomonas Reinhardtii Protein Complex Composition Sucrose Density Gradient Centrifugation SDS-PAGE Immunodetection Differential Labeling Comparative Analysis ANR1 CAS PGRL1 FNR Cyt F
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