Waiting
Traitement de la connexion…

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Cell-based Flow Cytometry Assay to Measure…
JoVE Journal
Immunology and Infection

Un abonnement à JoVE est nécessaire pour afficher ce contenu. Connectez-vous ou commencez votre essai gratuit.

JoVE Journal Immunology and Infection
Cell-based Flow Cytometry Assay to Measure Cytotoxic Activity
 

Cell-based Flow Cytometry Assay to Measure Cytotoxic Activity

Article DOI: 10.3791/51105-v • 10:14 min • December 17th, 2013
December 17th, 2013 •
1, 1, 1
1Vaccine and Gene Therapy Institute of Florida

Chapitres

résumé
Automatic Translation

This protocol describes a sensitive, cell-based cytotoxicity assay. By enumerating the decrease in frequency of live target CD4+ T cells in the presence of an increasing number of effector CD8+ T cells, this assay allows for the direct assessment of cytolytic activity of antigen-specific CD8+ T cells.

Tags

Keywords: Cell-based Flow Cytometry Assay Cytotoxic Activity CD8+ T Cells CD4+ T Cells CFSE Peptide MHC Tetramer Lytic Units
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter