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Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters
 

Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters

Article DOI: 10.3791/51153-v • 09:22 min • November 26th, 2013
November 26th, 2013 •
*1,3, *1, 1,2, 1
1The Lewis-Sigler Institute for Integrative Genomics, Princeton University, 2Department of Molecular Biology, Princeton University, 3Division of Chemistry and Chemical Engineering, California Institute of Technology
* These authors contributed equally

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This protocol describes an experimental procedure for the rapid construction of artificial transcription factors (ATFs) with cognate GFP reporters and quantification of the ATFs ability to stimulate GFP expression via flow cytometry.

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Keywords: Synthetic Biology Transcription Factors Gene Expression Reporter Systems GFP Homologous Recombination Artificial Transcription Factors Conditional Gene Expression
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