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Monitoring Intraspecies Competition in a Bacterial Cell Population by Cocultivation of Fluorescently Labelled Strains
 

Monitoring Intraspecies Competition in a Bacterial Cell Population by Cocultivation of Fluorescently Labelled Strains

Article DOI: 10.3791/51196-v 06:45 min January 18th, 2014
January 18th, 2014

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résumé

Bacteria may accumulate either detrimental or beneficial mutations during their lifetime. In a population of cells individuals that have accumulated beneficial mutations may rapidly outcompete their fellows. Here we present a simple procedure to visualize intraspecies competition in a bacterial cell population over time using fluorescently labeled individuals. 

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Keywords: Intraspecies Competition Bacterial Cell Population Fluorescently Labeled Strains Bacillus Subtilis Clonal Expansion Beneficial Mutations Detrimental Mutations Selection Genotype Shift Population Dynamics Cocultivation Microbial Competition
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