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En High Throughput MHC II Bindende Assay til kvantitativ analyse af peptidepitoper
 
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En High Throughput MHC II Bindende Assay til kvantitativ analyse af peptidepitoper

Article DOI: 10.3791/51308-v • 07:59 min • March 25th, 2014
March 25th, 2014 •
1, 2, 3, 1
1Thayer School of Engineering, Dartmouth College, 2Institute for Immunology and Informatics, University of Rhode Island, 3Department of Computer Science, Dartmouth College

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Biokemiske analyser med rekombinant humant MHC II-molekyler kan give hurtige, kvantitative indsigt i immunogen epitop identifikation, sletning eller design. Her er en bindende peptid-MHC II-analysen skaleret til 384-brønds plader beskrevet. Denne omkostningseffektive format skulle vise sig nyttig i de områder af protein deimmunization og vaccine design og udvikling.

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Biokemi Immunoassay Protein immunogenicitet MHC II T-celle epitop High Throughput Screen Deimmunization Vaccine Design
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