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JoVE Journal Neuroscience
Consensus Brain-derived Protein, Extraction Protocol for the Study of Human and Murine Brain Proteome Using Both 2D-DIGE and Mini 2DE Immunoblotting
 

Consensus Brain-derived Protein, Extraction Protocol for the Study of Human and Murine Brain Proteome Using Both 2D-DIGE and Mini 2DE Immunoblotting

Article DOI: 10.3791/51339-v • 10:51 min • April 10th, 2014
April 10th, 2014 •
1, 1,2, 1, 1, 1, 1, 1, 3, 4, 2, 3, 1, 1, 1, 1, 1
1Team Alzheimer & Tauopathies, Jean-Pierre Aubert Research Centre, Inserm UMR 837, 2EA 4308-Department of Reproductive Biology-Spermiology-CECOS, CHRU-Lille, 3EA2686-Laboratorie d'Immunologie, Faculté de Médecine - Pôle Recherche, 4Department of Neurology, CHRU-Lille

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résumé
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A common protein extraction protocol using urea/thiourea/SDS buffer for human and mice brain tissue allows indentification of proteins by 2D-DIGE and their subsequent characterization by mini 2DE immunoblotting. This method enables one to obtain more reproducible and reliable results from human biopsies and experimental models.

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Keywords: Brain Proteome 2D-DIGE Mini 2DE Immunoblotting Sample Preparation Human Post-mortem Mouse Brain Tissue Protein Aggregates Amyloid-beta Alpha-synuclein Neurodegenerative Diseases Biomarkers Therapeutic Targets
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