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Reverse Genetic Morpholino Approach Using Cardiac Ventricular Injection to Transfect Multiple Difficult-to-target Tissues in the Zebrafish Larva
 

Reverse Genetic Morpholino Approach Using Cardiac Ventricular Injection to Transfect Multiple Difficult-to-target Tissues in the Zebrafish Larva

Article DOI: 10.3791/51595-v • 08:22 min • June 17th, 2014
June 17th, 2014 •
1, 1
1DFG-Center for Regenerative Therapies Dresden, Technische Universität Dresden

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An adaptable reverse genetic method for zebrafish to assess gene function during later stages of development and physiological homeostasis such as tissue regeneration using intraventricular injections of gene-specific morpholinos.

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Keywords: Zebrafish Reverse Genetics Morpholino Cardiac Ventricular Injection Larval Tissue Gene Function Tissue Regeneration Tissue Homeostasis Embryogenesis Finfold Regeneration Vertebrate Regeneration
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