Waiting
Traitement de la connexion…

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / In Vivo 4-Dimensional Tracking of Hematopoietic…
JoVE Journal
Biology

Ce contenu est en libre accès.

JoVE Journal Biology
In Vivo 4-Dimensional Tracking of Hematopoietic Stem and Progenitor Cells in Adult Mouse Calvarial Bone Marrow
 

In Vivo 4-Dimensional Tracking of Hematopoietic Stem and Progenitor Cells in Adult Mouse Calvarial Bone Marrow

Article DOI: 10.3791/51683-v • 12:54 min • September 4th, 2014
September 4th, 2014 •
1, 2, 2
1Department of Life Science and Facility for Imaging by Light Microscopy, Imperial College London, 2Department of Life Sciences, Imperial College London

Chapitres

résumé
Automatic Translation

The nature of the interactions between hematopoietic stem and progenitor cells (HSPCs) and bone marrow niches is poorly understood. Custom hardware modifications and a multi-step acquisition protocol allow the use of two-photon and confocal microscopy to image ex vivo labeled HSPCs homed within bone marrow areas, tracking interactions and movement.

Tags

Keywords: Hematopoietic Stem Cells HSCs Hematopoietic Stem And Progenitor Cells HSPCs Bone Marrow Microenvironment In Vivo Imaging 4-dimensional Tracking Confocal Imaging Two-photon Imaging Osteoblastic Cells
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter