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Developmental Biology

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JoVE Journal Developmental Biology
Isolation of Human Mesenchymal Stem Cells and their Cultivation on the Porous Bone Matrix
 

Isolation of Human Mesenchymal Stem Cells and their Cultivation on the Porous Bone Matrix

Article DOI: 10.3791/51999-v • 09:00 min • February 9th, 2015
February 9th, 2015 •
1, 2, 2, 2, 1, 3, 4, 5, 6
1Depto. Materiales Metálicos y Cerámicos, Instituto de Investigaciones en Materiales, Universidad Nacional Autónoma de México (UNAM), 2Depto. of Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México (UNAM), 3Depto. Biología Celular y Tisular, Facultad de Medicina, Universidad Nacional Autónoma de México (UNAM), 4Depto de Biología Celular, Facultad de Química, Universidad Nacional Autónoma de México (UNAM), 5Depto. Genetica y Biología Molecular, Cinvestav-IPN, 6Subd. de Investigación, Centro Nacional de la Transfusión Sanguínea, Secretaria de Salud

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résumé
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Mesenchymal stem cells (MSCs) have a differentiation potential towards osteoblastic lineage when they are stimulated with soluble factors or specific biomaterials. This work presents a novel option for the delivery of MSCs from human amniotic membrane (AM-hMSCs) that employs the bovine bone matrix Nukbone (NKB) as a scaffold.

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Isolation Human Mesenchymal Stem Cells Cultivation Porous Bone Matrix Differentiation Potential Osteoblastic Lineage Soluble Factors Specific Biomaterials Delivery Human Amniotic Membrane Scaffold Repair Tissue Regeneration Techniques Host Tissue Biomaterials Cellular Scaffolds Topographical Characteristics Adhesion Proliferation Differentiation Injured Tissue Culture Technique
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