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Developmental Biology

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JoVE Journal Developmental Biology
Large-scale Zebrafish Embryonic Heart Dissection for Transcriptional Analysis
 

Large-scale Zebrafish Embryonic Heart Dissection for Transcriptional Analysis

Article DOI: 10.3791/52087-v • 10:00 min • January 12th, 2015
January 12th, 2015 •
*1,2,3, *1,2, 1,2,3
1Max Delbrück Center for Molecular Medicine, 2Institute of Biochemistry and Biology, University of Potsdam, 3Institute of Molecular Biology, Medizinische Hochschule Hannover
* These authors contributed equally

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résumé
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To analyse cardiac gene expression profiles during zebrafish heart development, total RNA has to be extracted from isolated hearts. Here, we present a protocol for collecting functional/beating hearts by rapid manual dissection from zebrafish embryos to obtain cardiac-specific mRNA.

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Keywords: Zebrafish Embryonic Heart Dissection Transcriptional Analysis Cardiac Transcriptome RNA Extraction Quantitative Real-time PCR Fluorescent Labeling Tissue Separation Fluidic Shear Force Filtration
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