Waiting
Traitement de la connexion…

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Bioengineering / Production and Targeting of Monovalent Quantum…
JoVE Journal
Bioengineering

Ce contenu est en libre accès.

JoVE Journal Bioengineering
Production and Targeting of Monovalent Quantum Dots
 

Production and Targeting of Monovalent Quantum Dots

Article DOI: 10.3791/52198-v • 10:16 min • October 23rd, 2014
October 23rd, 2014 •
*1,2,3, *4,5,6, 1,4,7, 1,7, 4,5,6,7
1Department of Otolaryngology, University of California, San Francisco, 2Department of Chemistry, University of California, Berkeley, 3Materials Science Division, Lawrence Berkeley National Laboratory, 4Department of Pharmaceutical Chemistry, University of California, San Francisco, 5Tetrad Graduate Program, University of California, San Francisco, 6Center for Systems and Synthetic Biology, University of California, San Francisco, 7Chemistry and Chemical Biology Graduate Program, University of California, San Francisco
* These authors contributed equally

Chapitres

résumé
Automatic Translation

We provide detailed instructions for the preparation of monovalent targeted quantum dots (mQDs) from phosphorothioate DNA of defined length. DNA wrapping occurs in high yield, and therefore, products do not require purification. We demonstrate the use of the SNAP tag to target mQDs to cell-surface receptors for live-cell imaging applications.

Tags

Keywords: Monovalent Quantum Dots MQDs CdSe/ZnS Core/shell QDs Phosphorothioate DNA PtDNA SNAP-tagged Notch Receptors Biological Targeting Live Cell Imaging Multivalent Quantum Dots Spatial Organization Of Biomolecules
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter