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Immunology and Infection

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JoVE Journal Immunology and Infection
Nucleocapsid Annealing-Mediated Electrophoresis (NAME) Assay Allows the Rapid Identification of HIV-1 Nucleocapsid Inhibitors
 

Nucleocapsid Annealing-Mediated Electrophoresis (NAME) Assay Allows the Rapid Identification of HIV-1 Nucleocapsid Inhibitors

Article DOI: 10.3791/52474-v • 08:33 min • January 19th, 2015
January 19th, 2015 •
1, 1, 2, 1
1Department of Pharmaceutical and Pharmacological Sciences, University of Padova, 2The RNA Institute, SUNY Albany

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This protocol describes NAME, an assay that allows the rapid identification of molecules able to inhibit in vitro the chaperone activities of HIV-1 nucleocapsid protein.

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Keywords: Nucleocapsid Annealing-Mediated Electrophoresis (NAME) Assay HIV-1 Nucleocapsid Protein (NC) RNA/DNA Folding Antiviral Drug Development NC Chaperone Activity Inhibition Threading Intercalator PAGE Analysis
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