Waiting
Traitement de la connexion…

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Bioengineering / Isolation of Primary Human Colon Tumor Cells from …
JoVE Journal
Bioengineering

Un abonnement à JoVE est nécessaire pour afficher ce contenu. Connectez-vous ou commencez votre essai gratuit.

JoVE Journal Bioengineering
Isolation of Primary Human Colon Tumor Cells from Surgical Tissues and Culturing Them Directly on Soft Elastic Substrates for Traction Cytometry
 

Isolation of Primary Human Colon Tumor Cells from Surgical Tissues and Culturing Them Directly on Soft Elastic Substrates for Traction Cytometry

Article DOI: 10.3791/52532-v • 09:28 min • June 4th, 2015
June 4th, 2015 •
1, 1, 2,3, 2,3, 1,4
1Department of Mechanical Science and Engineering, University of Illinois at Urbana-Champaign, 2College of Medicine, University of Illinois at Urbana-Champaign, 3Provena Covenant Medical Centre, 4Micro and Nanotechnology Laboratory, University of Illinois at Urbana-Champaign

Chapitres

résumé
Automatic Translation

A protocol is described to extract primary human cells from surgical colon tumor and normal tissues. The isolated cells are then cultured on soft elastic substrates (polyacrylamide hydrogels) functionalized by an extracellular matrix protein, and embedded with fluorescent microbeads. Traction cytometry is performed to assess cellular contractile stresses.

Tags

Keywords: Primary Human Colon Tumor Cells Surgical Tissues Soft Elastic Substrates Traction Cytometry Matrix Mechanical Stiffness Adhesion Receptors Signal Transduction Cytoskeleton Molecular Motors Mechanosensitivity Immortalized Cell Lines Murine Derived Primary Cells Polyacrylamide Hydrogels Extracellular Matrix Fibronectin Traction Assay Cellular Contractile Stresses Immunofluorescence Microscopy Cell Morphology Cytoskeleton Organization Vinculin Focal Adhesions
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter