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JoVE Journal Developmental Biology
Automated Quantification of Hematopoietic Cell – Stromal Cell Interactions in Histological Images of Undecalcified Bone
 

Automated Quantification of Hematopoietic Cell – Stromal Cell Interactions in Histological Images of Undecalcified Bone

Article DOI: 10.3791/52544-v • 09:31 min • April 8th, 2015
April 8th, 2015 •
1, 2,3, 4, 2, 1,5
1Immunodynamics, German Rheumatism Research Center, a Leibniz Institute, 2Biophysical Analytics, German Rheumatism Research Center, a Leibniz Institute, 3Max-Delbrück Center for Molecular Medicine, 4Wimasis GmbH, 5Immunodynamics and Intravital Imaging, Charité - University of Medicine

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résumé
Automatic Translation

A strategy to quantitatively analyze histological data in the bone marrow is presented. Confocal microscopy of fluorescently labeled cells in tissue sections results in 2-dimensional images, which are automatically analyzed. Co-localization analyses of different cell types are compared to data from simulated images, giving quantitative information about cellular interactions.

Tags

Automated Quantification Hematopoietic Cell Stromal Cell Histological Images Undecalcified Bone Confocal Microscopy Cellular Localization Manual Counting Interrater Reliability Co-localization Unbiased Quantification Bone Marrow Sample Preparation Staining Protocol High-resolution Images Analysis Workflow Hematopoietic Cell Types Non-hematopoietic Cell Types Neighborhood Analysis
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