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A Fast and Reliable Pipeline for Bacterial Transcriptome Analysis Case study
 

A Fast and Reliable Pipeline for Bacterial Transcriptome Analysis Case study: Serine-dependent Gene Regulation in Streptococcus pneumoniae

Article DOI: 10.3791/52649-v • 10:18 min • April 25th, 2015
April 25th, 2015 •
1,2, 1,2, 1
1Department of Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, 2Department of Bioinformatics and Biotechnology, Government College University Faisalabad

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This manuscript describes the use of state-of-the-art technology provided by DNA-microarrays. Microarrays provide an overview of the transcriptomic changes in bacteria incurred under a specific condition. Moreover, we highlight the ease by which large amounts of data can be analyzed by using convenient in-house developed software packages.

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Bacterial Transcriptome Analysis Serine-dependent Gene Regulation Gene Expression DNA Microarrays Research Technology Global Gene Expression Novel Genes Transcription Factor Binding Sites Transcriptional Activity Cell Harvest DNA Microarray Analysis Streptococcus Pneumoniae L-serine Concentrations Total RNA Isolation Macaloid Method RNA Quality Check Kit CDNA Preparation
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