Waiting
Traitement de la connexion…

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / High-throughput Quantitative Real-time RT-PCR…
JoVE Journal
Immunology and Infection

Ce contenu est en libre accès.

JoVE Journal Immunology and Infection
High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes
 

High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes

Article DOI: 10.3791/52650-v • 10:00 min • March 24th, 2015
March 24th, 2015 •
1, 1, 1, 1, 1, 1, 1, 1, 2, 2, 1
1Center for Biologics Evaluation and Research, US Food and Drug Administration, 2Center for Drug Evaluation and Research, US Food and Drug Administration

Chapitres

résumé
Automatic Translation

This protocol describes a high-throughput qRT-PCR assay for the analysis of type I and III IFN expression signatures. The assay discriminates single base pair differences between the highly similar transcripts of these genes. Through batch assembly and robotic pipetting, the assays are consistent and reproducible.

Tags

Keywords: QRT-PCR Interferon Subtypes Type I Interferons Type III Interferons LNA Probes Molecular Beacon Probes Standards Automated Pipetting Plate Drying Gene Expression Profiling
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter