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Measurement of Extracellular Ion Fluxes Using the Ion-selective Self-referencing Microelectrode Technique
 

Measurement of Extracellular Ion Fluxes Using the Ion-selective Self-referencing Microelectrode Technique

Article DOI: 10.3791/52782-v • 09:18 min • May 3rd, 2015
May 3rd, 2015 •
1, 1, 1,2, 3, 1,4
1Department of Dermatology, Institute for Regenerative Cures, University of California, Davis, 2Departamento de Biologia, Centro de Biologia Molecular e Ambiental, Universidade do Minho, 3Department of Neurology and Center for Neuroscience, University of California, Davis Imaging of Dementia and Aging Laboratory, 4Department of Ophthalmology, Institute for Regenerative Cures, University of California, Davis

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Transporters in cell membranes allow differential segregation of ions across cell membranes or cell layers and play crucial roles during tissue physiology, repair and pathology. We describe the ion-selective self-referencing microelectrode that allows the measurement of specific ion fluxes at single cells and tissues in vivo.

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Extracellular Ion Fluxes Ion-selective Self-referencing Microelectrode Technique Cell Membrane Differential Distribution Of Ions Selective Permeability Ion Transport Tissue Physiology Signaling Cues Calcium Flux Potassium Flux Sodium Flux Microelectrode Ionophore Cocktail Ion Concentration Electric Potential
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