JoVE Journal
Immunology and Infection
Immunology and Infection
Ce contenu est en libre accès.
Chapitres
résumé
While high resolution melting analysis offers the ability to differentiate between single nucleotide polymorphisms in a heterogeneous population, mutant allele amplification bias can increase its ability to detect alleles present at relatively low percentages within a sample. This protocol describes improvements that improve the sensitivity of high resolution melting analysis.