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Induction and Assessment of Ischemia-reperfusion Injury in Langendorff-perfused Rat Hearts
 

Induction and Assessment of Ischemia-reperfusion Injury in Langendorff-perfused Rat Hearts

Article DOI: 10.3791/52908-v • 07:58 min • July 27th, 2015
July 27th, 2015 •
1, 1, 1,2
1Department of Medicine/Cardiology, Gazes Cardiac Research Institute, Medical University of South Carolina, 2Ralph H. Johnson VA Medical Center, Medical University of South Carolina

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Automatic Translation

The isolated rat heart is an enduring model for ischemia reperfusion injury. Here, we describe the process of harvesting the beating heart from a rat via in situ aortic cannulation, Langendorff perfusion of the heart, simulated ischemia-reperfusion injury, and infarct staining to confirm the extent of ischemic insult.

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Induction Assessment Ischemia-reperfusion Injury Langendorff-perfused Rat Hearts Biochemical Events Treatment Options Myocardial Infarction Cardiac Complications Thoracic Surgery Drugs Preconditioning Clinical Trials Isolated Heart Model Functional Effects Pharmacological Interventions Aortic Cannulation Non-preconditioned Myocardium Pressure Generation Simulation Of Ischemic Injury Perfusion Buffer Flow Reperfusion Modulated Duration Biochemical Events
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