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JoVE Journal Neuroscience
Limbal Approach-Subretinal Injection of Viral Vectors for Gene Therapy in Mice Retinal Pigment Epithelium
 

Limbal Approach-Subretinal Injection of Viral Vectors for Gene Therapy in Mice Retinal Pigment Epithelium

Article DOI: 10.3791/53030-v • 06:48 min • August 7th, 2015
August 7th, 2015 •
1,2, 2, 3, 1,2,4
1Department of Biomedical Sciences, Seoul National University College of Medicine, 2FARB Laboratory, Biomedical Research Institute, Seoul National University Hospital, 3College of Life Sciences, Gwangju Institute of Science and Technology, 4Department of Ophthalmology, Seoul National University College of Medicine

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Subretinal injection is a surgical technique for effective gene delivery to retinal pigment epithelium in the mouse eye. Here we describe an easy and replicable method for subretinal injection of viral vectors to retinal pigment epithelium in experimental mice.

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Keywords: Gene Therapy Viral Vector Subretinal Injection Retinal Pigment Epithelium RPE Mice Ocular Gene Therapy Intravitreal Injection Flat-mount Gene Transduction
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