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Chapitres
- 00:05Titre
- 02:08Preparation of Materials and Supports
- 04:18Dissection of Embryos, Tissue Dissociation, and Cell Plating
- 06:323D Neuronal Network Construction
- 07:28Results: Examples of 3D Networks Captured by Confocal Microscopy and by Electrophysiological Recordings
- 08:24Conclusion
In this work, a novel experimental model in which 3D neuronal cultures are coupled to planar Micro-Electrode Arrays (MEAs) is presented. 3D networks are built by seeding neurons in a scaffold made up of glass microbeads on which neurons grow and form interconnected 3D structures.
