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Developmental Biology

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JoVE Journal Developmental Biology
Accurate and Phenol Free DNA Sexing of Day 30 Porcine Embryos by PCR
 

Accurate and Phenol Free DNA Sexing of Day 30 Porcine Embryos by PCR

Article DOI: 10.3791/53301-v • 10:16 min • February 14th, 2016
February 14th, 2016 •
*1, *1, 1
1Department of Agricultural, Food and Nutritional Science, University of Alberta
* These authors contributed equally

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résumé
Automatic Translation

This protocol describes an accurate, inexpensive, rapid and non-toxic method to determine the sex of Day 30 porcine embryo using PCR method after grinding an embryo into powder without phenol chloroform extraction and DNA column purification.

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Keywords: DNA Sexing PCR Porcine Embryos Phenol-free Reproductive Physiology Sex Ratio DNA Purification Embryo Samples Liquid Nitrogen Sodium Hydroxide DNA Lysate
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