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The c-FOS Protein Immunohistological Detection
 

The c-FOS Protein Immunohistological Detection: A Useful Tool As a Marker of Central Pathways Involved in Specific Physiological Responses In Vivo and Ex Vivo

Article DOI: 10.3791/53613-v • 05:44 min • April 25th, 2016
April 25th, 2016 •
1,2, 1,3, 1,3,4, 2, 1,3, 2, 1,3
1Sorbonne Paris Cité, Laboratory “Hypoxia & Lung” EA2363, University Paris 13, 2UPMC Univ Paris 06, INSERM, UMR_S1158 Neurophysiologie Respiratoire Expérimentale et Clinique, Sorbonne Universités, 3Laboratory of Excellence GR-Ex, 4Laboratory MOVE (EA 6314), University of Poitiers

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Here, we present a protocol based on c-FOS protein immunohistological detection, a classical technique used for the identification of neuronal populations involved in specific physiological responses in vivo and ex vivo.

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C-FOS Protein Immunohistological Detection Marker Central Pathways Physiological Responses In Vivo Ex Vivo Neuronal Activity Brain Mapping Brain Regions Neurophysiological Field Brain Tissues C-FOS Gene Stimulation Quantification Test Simulation Coronal Brain Stem Sections Wash PBS Endogenous Peroxidase Activity Hydrogen Peroxide Block Nonspecific Binding Sites Goat Serum PBST Polyclonal Antibody
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