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Neuroscience

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JoVE Journal Neuroscience
Sex Differences in Mouse Hippocampal Astrocytes after In-Vitro Ischemia
 

Sex Differences in Mouse Hippocampal Astrocytes after In-Vitro Ischemia

Article DOI: 10.3791/53695-v • 08:32 min • October 25th, 2016
October 25th, 2016 •
1, 2, 1, 1, 1,3, 1,3
1Waisman Center, University of Wisconsin, 2Department of Neurological Surgery, University of Wisconsin, 3Department of Pediatrics, University of Wisconsin

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Astrocytes are one of the most important key players in the central nervous system (CNS). Here, we are reporting a practical method of sexed hippocampal astrocyte culture protocol in order to study the mechanisms underlying the astrocyte function in male and female neonate pups after in-vitro ischemia.

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Sex Differences Mouse Hippocampal Astrocytes In-vitro Ischemia Enriched Cultures Oxygen And Glucose Deprivation Reoxygenation Hypoxic Ischemia Mechanistic Questions Translational Questions Developing Male And Female Brains Brain Dissection Newborn Mouse Pup Posterior To Anterior Midline Incision Cranium Removal Brainstem Removal Dissecting Dish Hemispheres Separation Cerebral Hemispheres Peeling Midbrain And Thalamic Tissue Removal Hippocampus Removal Meninges And Fibroblasts Cleaning
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