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Immunology and Infection

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JoVE Journal Immunology and Infection
Myeloid Cell Isolation from Mouse Skin and Draining Lymph Node Following Intradermal Immunization with Live Attenuated Plasmodium Sporozoites
 

Myeloid Cell Isolation from Mouse Skin and Draining Lymph Node Following Intradermal Immunization with Live Attenuated Plasmodium Sporozoites

Article DOI: 10.3791/53796-v • 08:46 min • May 18th, 2016
May 18th, 2016 •
1, 2, 1, 1
1Unité de Biologie et Génétique du Paludisme, Institut Pasteur, 2Unité dImmunobiologie des Cellules Dendritiques, Institut Pasteur

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résumé
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We describe here a protocol for isolating myeloid cells from mouse skin and draining lymph node following intradermal injection of Plasmodium sporozoites. Flow cytometry of collected cells provides a reliable assay to characterize the skin and draining lymph node inflammatory response to the parasite.

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Keywords: Myeloid Cell Isolation Mouse Skin Draining Lymph Node Intradermal Immunization Live Attenuated Plasmodium Sporozoites Malaria Innate Immune Response Radiation-attenuated Parasites Mosquito Salivary Glands Sporozoite Injection Ear Pinna Dermis
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