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Developmental Biology

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JoVE Journal Developmental Biology
Multi-target Chromogenic Whole-mount In Situ Hybridization for Comparing Gene Expression Domains in Drosophila Embryos
 

Multi-target Chromogenic Whole-mount In Situ Hybridization for Comparing Gene Expression Domains in Drosophila Embryos

Article DOI: 10.3791/53830-v • 10:17 min • January 31st, 2016
January 31st, 2016 •
1, 1, 1, 1
1Department of Molecular Biosciences, The Wenner-Gren Institute (MBW), Stockholm University

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We describe a multi-target chromogenic whole-mount in situ hybridization (MC-WISH) procedure in intact Drosophila embryos allowing simultaneous and specific detection of three different mRNA distribution patterns by contrasting color precipitates.

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Keywords: Multi-target Chromogenic Whole-mount In Situ Hybridization Gene Expression Drosophila Embryos MRNA Localization Multi-color Gene Regulatory Interactions Embryonic Development Organogenesis
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